Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Inflammasome activation as a key driver of acetaminophen induced hepatotoxicity: Mechanisms and emerging therapeutics EurekAlert! Oxidative stress mediates end organ damage in a novel model of acetaminophen toxicity in Drosophila Scientific Reports Can Tylenol (acetaminophen) deplete glutathione levels in the body? Recurrent Pyroglutamic Acidosis Related to Therapeutic Acetaminophen ScienceDirect